Ekeler reportedly wore a brace on the knee and walked on crutches after game, a 38-31 win by the host Tampa Bay Buccaneers. He finished with two carries for 12 yards and one reception for 2 yards.
The Chargers plan to have Ekeler undergo an MRI exam on Monday to determine the severity of the injury.
The 25-year-old Ekeler burst onto the scene last season with 1,550 yards from scrimmage and 11 touchdowns. He has 23 career touchdowns -- nine on the ground and 14 receiving.
The Chargers are scheduled to visit the New Orleans Saints next week.
--Field Level Media