England will be hoping to go far into the knockout stages, having made the semi-finals of the last World Cup and the final of Euro 2020.
The sides met in the group stages of Euro 2016, a match which England went on to win 2-1, requiring a 91st-minute winner from Daniel Sturridge. Gareth Bale got Wales's goal.
Wales must beat England in their final group game to have any hope of avoiding an early elimination, a fate already suffered by hosts Qatar just two games into their World Cup campaign.
When is the match?
The match between England and Wales takes place at 7pm UK time on Tuesday, November 29 (10pm in Qatar and 2pm EST).
Where will it take place?
The game will be played in the Ahmad Bin Ali Stadium (also known as the Al-Rayyan Stadium) in Al-Rayyan. The 40,000 seater ground was inaugurated on 18 December 2020 when it hosted the Amir Cup final, and is the home of Al Rayyan Sports Club.
It is the host location for all three of Wales's group stage matches, which could give Robert Page's men a slight advantage through familiarity.
What TV channel is it on?
The match will be shown live on BBC One. You can also stream it on BBC iPlayer.
England and Wales have played each other 103 times – the first fixture taking place in 1879, and the most recent in 2020.
England have won 68, drawn 21 and lost just 14. You have to go back to 1984 for Wales's last victory against England. The Three Lions have won the last six encounters.
What are they saying?
Gareth Bale and Aaron Ramsey, so often the architects of Wales' successes down the years, were both disappointing in their 2-0 defeat to Iran and they now need a victory over England on Tuesday to have any hope of reaching the knockout stages.
Head coach Rob Page even appeared to refer to the England game as Wales' "final" match of the tournament, underlining how difficult the road ahead is for the Dragons from here.
"This is a hard one to take," Page said. "I've said to them before every game: 'Go and show the world what you show us every day,' and that is not a true reflection of that team.
"They got their true rewards today, they deserved that defeat. We fell well below the standards that got us to the World Cup, if we'd met those standards we'd have been alright. And if you do that in top competitions, you get punished."
Asked about the England game, Page said: "We want to finish the competition on a high. It's out of our hands for going through, but we want to finish with a good performance and a win.
"We're low at the moment, but we'll get them back up tomorrow for a tough game to finish with."
England to win 4/9
Wales to win 13/2