The injury comes as Barnes, who did not practise Tuesday, was rounding into form after a string of disappointing games.
The 2021-22 rookie of the year had 28 points, 11 rebounds and nine assists in Toronto's 124-122 overtime loss at Atlanta on Saturday.
Barnes joins a growing injury list in Toronto that includes star forward Pascal Siakam, who has been out since sustaining an adductor injury in a Nov. 4 loss in Dallas.
Guard Dalano Banton is questionable with a non-COVID illness.
The Raptors will get a starting player back for Wednesday, with guard Gary Trent Jr. returning from a hip injury and illness.
This report by The Canadian Press was first published Nov. 22, 2022.
The Canadian Press